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KMID : 0624620170500050263
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BMB Reports
2017 Volume.50 No. 5 p.263 ~ p.268
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Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity
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Lee Moon-Hee
Jang Jong-Hwa
Yoon Gun-Young
Lee Seung-Jun
Lee Min-Goo
Kang Tae-Heung
Han Hee-Dong
Kim Hyuk-Soon
Choi Wahn-Soo
Park Won-Sun
Park Yeong-Min
Jung In-Duk
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Abstract
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β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.
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KEYWORD
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Antitumor immunity, Dendritic cells, Natural killer cells, Neoagarohexaose, Toll-like receptor 4
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